Anti-Cannabinoid Receptor I/CNR1 Antibody Picoband™

Product Name

Anti-Cannabinoid Receptor I/CNR1 Antibody Picoband™

View all Cannabinoid R1/CB1/CNR1 Antibodies

SKU/Catalog Number

A01291-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cannabinoid Receptor I/CNR1 Antibody Picoband™ catalog # A01291-1. Tested in ELISA, Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cannabinoid Receptor I/CNR1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01291-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Cannabinoid Receptor I recombinant protein (Position: M1-Q75).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01291-1 is reactive to CNR1 in Human, Mouse, Rat

Applications

A01291-1 is guaranteed for ELISA, Flow Cytometry, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

52.858kDa

Background of Cannabinoid R1/CB1/CNR1

The cannabinoid receptor type 1, often abbreviated as CB1, is a G protein-coupled cannabinoid receptor located primarily in the central and peripheral nervous system. This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x10⁶ cells
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Cannabinoid Receptor I using anti-Cannabinoid Receptor I antibody (A01291-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human 22RV1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cannabinoid Receptor I antigen affinity purified polyclonal antibody (Catalog # A01291-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cannabinoid Receptor I at approximately 60KD. The expected band size for Cannabinoid Receptor I is at 53KD.

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Figure 2. IHC analysis of Cannabinoid Receptor I using anti-Cannabinoid Receptor I antibody (A01291-1).
Cannabinoid Receptor I was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cannabinoid Receptor I Antibody (A01291-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of Cannabinoid Receptor I using anti-Cannabinoid Receptor I antibody (A01291-1).
Cannabinoid Receptor I was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cannabinoid Receptor I Antibody (A01291-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 4. Flow Cytometry analysis of Hela cells using anti-CNR1 antibody (A01291-1).
Overlay histogram showing Hela cells stained with A01291-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CNR1 Antibody (A01291-1,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For CNR1 (Source: Uniprot.org, NCBI)

Alternative Names

CANN6; CANN6CB1R; Cannabinoid R1; cannabinoid receptor 1 (brain); CannabinoidR1; CB1; CB1A; CB1K5; CB1R; CB-R; CB-RCB1cannabinoid receptor 1; central cannabinoid receptor; CNR; CNR1; SKR6R CNR1 CANN6, CB-R, CB1, CB1A, CB1K5, CB1R, CNR cannabinoid receptor 1 cannabinoid receptor 1|cannabinoid receptor 1 (brain)|central cannabinoid receptor

For more info on CNR1, check out the CNR1 Infographic

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In this infographic, you will see the following information for CNR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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